Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: The prognostic HRs of individual LGALS members in all breast cancers in www.kmplot.com. Only 9 members (LGALS1, LGALS2, LGALS3, LGALS4, LGALS8, LGALS10, LGALS12, LGALS13, and LGALS14) were found on www.kmplot.com. Overexpression of LGALS2 mRNA was correlated with satisfactory overall survival in all breast cancer patients HR: Hazard ratio; CI: confidence intervals.

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Over Expression

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: The prognostic value of LGALS member expression in breast cancer with Affymetrix IDs. The prognostic survival curves of LGALS1 (A) 201105_at, LGALS2 (B) 208450_at, LGALS3 (C) 208949_s_at, LGALS4 (D) 204272_at, LGALS8 (E) 210731_s_at, LGALS10 (F) 206207_at, LGALS12 (G) 223828_s_at, LGALS13 (H) 220440_at, and LGALS14 (I) 220158_at were plotted for all breast cancer patients (n=943 for LGALS12 and n=1879 for the rest of all LGALS members).

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: Correlation of LGALS expression level with OS in different pathological grades in breast cancer patients

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: Correlation of LGALS expression level with OS in different clinical stages in breast cancer patients

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: Correlation of LGALS expression level with OS in breast cancer patients with different HER2 statuses

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: Correlation of LGALS expression level with OS in breast cancer patients with different lymph node status

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: Correlation of AQP gene expression with OS in breast cancer patients with TP53 mutation status

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Gene Expression, Mutagenesis

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: The table shows the basic information of the IHC

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques:

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: Analysis of (A) LGALS2 mRNA expression levels in breast cancer cells and normal breast cancer cells using qRT-PCR. The expression of LGALS2 was detected in (B). The quantitative histogram of LGALS2 in breast cancer cells and normal breast cells using western blot analysis and (C) western blot bands. The human breast tumour cell lines T47D, MDA-MB-231, and MCF-7 and the human normal breast epithelial cell line MCF10A were used. Data are presented as the mean ± standard deviation from triplicate experiments. (*P<0.05; **P<0.01; ***P<0.001).

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation

Journal: American Journal of Translational Research

Article Title: Overexpression of galectin2 (LGALS2) predicts a better prognosis in human breast cancer

doi:

Figure Lengend Snippet: The associated protein expression of LGALS2 in human breast tissues was detected by immunohistochemistry (SP staining, ×200). A and B. Representative images and scores of IHC for LGALS2 in human breast cancer tissue and matched normal breast tissue. Values were expressed as the mean +/- SD. *P<0.05.

Article Snippet: The membranes were incubated with primary antibodies against LGALS2 (1:4000, mouse anti-human, B Boster, China) using a 5% skim milk blocking agent, and anti-GAPDH (1:2000, mouse anti-human, Abbkine, China) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry, Staining